The intent of this report is to identify unknown #12 the conclusion of unknown #12's identity was reached after a series of tests and subcultures were conducted using aseptic techniques aseptic techniques are necessary in order to avoid contamination of the culture being examined dr burne. Introduction the purpose to this lab was to isolate and identify two unknown bacteria from a mixed culture provided to us by our instructor this study was done by applying all of the methods that have been instructed on thus far in microbiology laboratory class. Capabilities of the organism are studied in more detail identifying an unknown is a fascinating process in clinical medicine often times a pathogen is isolated from. Unknown lab report unknown organism #6 ann le (phuoc) may 6, 2010 dr carrington microbiology lab- mw 12:50 le 1 i introduction my unknown organism #6 is morganella morganii, which is a gram-negative bacillus rods commonly found in the environment and also in the intestinal tracts of humans, mammals, and reptiles as a normal flora.
The results from the gram stain of unknown number 24 showed that unknown number 24 has bacillus shaped cell morphology, holding a dark-pink color confirming it to be a gram negative organism indeed in both gram-positive and gram-negative the cell walls house peptidoglycan, which is a blend of carbohydrates and amino-acids. Unknown bacteria lab report the purpose to this lab was to isolate and identify two unknown bacteria from a mixed culture provided to us by our instructor this study was done by applying all of the methods that have been instructed on thus far in microbiology laboratory class. General unknown reports in microbiology are written in scientific format scientific writing is written differently from other types of writing the results of the exercise or experiment are what are being showcased, not the writing the purpose of scientific writing is not to entertain, but to. No month passes without some new micro-organisms being discovered, many of them having new, hitherto unknown properties an example is the discovery of a new form of phototrophy in two widespread aerobic bacteria in surface waters of oceans ( beja et al , 2000 kolber et al , 2000 .
How to write a microbiology lab report whether you're studying microbiology in high school or as a college student, you'll need to write a number of lab reports the lab-report genre does have several sections you'll need to meet in your. Unknown report #34 11/12/09 biol 2133 fall 2009 november 12, 2009 unknown report purpose: the purpose of the unknown report is to be able to identify an unknown bacteria culture using a series of differential tests. Unknown lab report production by organism result from the mannitol fermentation test it was decided that unknown #545 was enterococcus faecalis.
Dubay takes you into his ib biology classroom for this unedited introduction he sets the context for the use of identification keys and stresses that meanin. Identification of unknown bacteria is one of the major responsibilities of the microbiologists samples of blood, tissue, food, water and cosmetics are examined daily in laboratories throughout the world for the presence of contaminating microorganisms in addition, industrial organisations are. Staphylococcus is a genus of gram +, nonspore-forming cocci belonging to the family micrococcaceae that are often found as normal human microbiota of the skin and nasal cavity there are five organisms to consider as potential human pathogens in this genus: s aureus, s. There are numerous reasons for identifying unknown bacteria some of these organisms have distinct qualities that set them apart from one another, such as the exposure to certain environments.
Unknown bacteria are first collected by swabbing surfaces around and near the lab, then streaked on sterile lb agar plates and grown overnight in an incubator. The importance of identifying unknown bacteria is very important for one reason, bacteria does not stay in the same form, it's mutating all the time i believe, if doctors need to treat some bacteria with antibiotics, they need to identify first, what type of bacteria they dealing with. The unknown #123 contained two different specimen of bacteria, one being a gram positive bacteria and one being a gram negative bacteria the first unknown in #123 found to be a gram negative bacteria, was identified as pseudomonas aeruginosa. Interpret tests conducted to identify a certain organism • to demonstrate skill in coordinating the usual laboratory work with that of identifying an unknown organism. You must print a copyof the rubric for both case study #1 and case study #2 and staple them to your lab 14 lab report before you submit the report to your instructor case study #2, unknown #3 a 57 year old diabetic male hospitalized following hip replacement surgery has had an indwelling urinary catheter inserted for 8 days.
Table i and flowchart i list all the tests, purposes, results, and order the tests where done for the gram positive bacteria using the unknown 109 tube table ii and flowchart ii list all the tests, purposes, results, and order the tests where done for the gram negative bacteria using the alternative #4 tube. Unknown, it is customary to write, a trypticase soy agar (tsa) plate was used to isolate the unknown it is also customary to write in the past tense for most of the report. Unknown lab report unknown number 103 (staphylococcus aureus and klebsiella pneumoniae) michelle gudorp general microbiology spring 2013 introduction it is important to understand why a person would want to identify between different bacteria. Unknown organism, c 14 appeared pink in color following the gram stain experiment, proving it is a gram negative bacterium this finding helped to eliminate the gram positive.
In lab, we have to identify two unknown organisms in a sample of nutrient broth i gram stained it and found gram+ cocci, and gram- rods i have to have a plan on how to further identify the organisms and i really have no idea where to start. Unknown bacteria lab report introduction the purpose to this lab was to isolate and identify two unknown bacteria from a mixed culture provided to us by our instructor.
The unknown bacteria will be one of the following: enterococcus faecalis, staphylococcus saprophyticus, escherichia coli, enterobacter aerogenes, proteus vulgaris, salmonella [i assume typhimurium], or shigella [either flexneri or sonnei, we used both in our lab during the semester. October 10, 2007 microbiology lab report the unknown received was determined as staphylococcus epidermidis this identification was made by testing the following: gram stain, mannitol salt agar (msa) and blood agar (bap) biochemical tests, the catalase test, bile esculin test, and looking at the colony morphology. That's right you have more than one organism in your tube the first step is to streak your unknown broth for isolation on a tsa plate and incubate it for 24-48 hours. The ability of an organism to reduce sulfur-containing compounds to hydrogen sulfide can be another test for identifying unknown organisms such as certain proteus and salmonella to test for hydrogen sulfide production, a medium with a sulfur-containing compound and iron salts is inoculated and incubated.